\u3010\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u5b9a\u91cfPCR\u6cd5\u3068\u306f\u3011<\/p>\n
\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u5b9a\u91cfPCR\u6cd5\u306f\u3001PCR\u306b\u3088\u308b\u5897\u5e45\u53cd\u5fdc\u3092\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u306b\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u3057\u3001\u30b5\u30f3\u30d7\u30eb\u4e2d\u306b\u5b58\u5728\u3059\u308bDNA\u3001RNA\u306e\u91cf\u3092\u5b9a\u91cf\u7684\u306b\u6e2c\u5b9a\u3059\u308b\u65b9\u6cd5\u3067\u3059\u3002\u3042\u3089\u304b\u3058\u3081\u6c7a\u3081\u3089\u308c\u305f\u30b5\u30a4\u30af\u30eb\u6570\u3067\u306e\u30d0\u30f3\u30c9\u306e\u6709\u7121\uff08\u6fc3\u6de1\uff09\u306b\u3088\u308aPCR\u7523\u7269\u91cf\u3092\u6e2c\u5b9a\u3059\u308bPCR(RT-PCR)\u6cd5\u306b\u6bd4\u3079\u3066\u3001\uff081\uff09\u9ad8\u3044\u5b9a\u91cf\u6027\u3001\uff082\uff09\u9ad8\u611f\u5ea6\u3001\uff083\uff09\u5e83\u3044\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8\u3001\uff084\uff09\u9ad8\u3044\u518d\u73fe\u6027\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n
\u307e\u305f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u5b9a\u91cfPCR\u306b\u306f\u4ee5\u4e0b\u306b\u793a\u3059\u3088\u3046\u306b\u3044\u304f\u3064\u304b\u65b9\u6cd5\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n
1.\u7d76\u5bfe\u5b9a\u91cf
\n\u65e2\u77e5\u306e\u30b3\u30d4\u30fc\u6570\u3092\u542b\u3093\u3060\u6a19\u6e96\u30b5\u30f3\u30d7\u30eb\u3092\u7528\u3044\u3066\u691c\u91cf\u7dda\u3092\u4f5c\u6210\u3057\u3001\u6e2c\u5b9a\u5bfe\u8c61\u30b5\u30f3\u30d7\u30eb\u306e\u30b3\u30d4\u30fc\u6570\uff08\u7d76\u5bfe\u91cf\uff09\u3092\u6e2c\u5b9a\u3059\u308b\u65b9\u6cd5\u3067\u3059\u3002 <\/p>\n
2.\u76f8\u5bfe\u5b9a\u91cf\uff08\u6a19\u6e96\u30b5\u30f3\u30d7\u30eb\u306b\u3088\u308b\u691c\u91cf\u7dda\u6cd5\uff09
\n\u76ee\u7684\u306e\u907a\u4f1d\u5b50\uff08\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\uff09\u3068\u5185\u90e8\u6a19\u6e96\u907a\u4f1d\u5b50\uff08\u30ea\u30d5\u30a1\u30ec\u30f3\u30b9\u907a\u4f1d\u5b50\uff09\u306e\u76f8\u5bfe\u5024\u3092\u7b97\u51fa\u3057\u3001\u30b5\u30f3\u30d7\u30eb\u9593\u306e\u6bd4\u8f03\u3092\u884c\u3046\u65b9\u6cd5\u3067\u3059\u3002\u6a19\u6e96\u30b5\u30f3\u30d7\u30eb\u3092\u7528\u3044\u3066\u3001\u907a\u4f1d\u5b50\u3054\u3068\u306b\u691c\u91cf\u7dda\u3092\u4f5c\u6210\uff08\u5185\u90e8\u6a19\u6e96\u907a\u4f1d\u5b50\u3082\u542b\u3080\uff09\u3057\u3001\u307e\u305f\u3001\u30b5\u30f3\u30d7\u30eb\u306b\u3088\u308a\u6700\u9069\u306a\u5185\u90e8\u6a19\u6e96\u907a\u4f1d\u5b50\u3092\u9078\u629e\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002 <\/p>\n
3.\u6bd4\u8f03Ct\u6cd5\uff08\u0394\u0394Ct\u6cd5\uff09
\n\u6bd4\u8f03Ct\u6cd5\u306f\u57fa\u6e96\u3068\u306a\u308b\u30b5\u30f3\u30d7\u30eb\u3068\u6bd4\u8f03\u3057\u3066\u3001\u672a\u77e5\u30b5\u30f3\u30d7\u30eb\u304c\u4f55\u30b5\u30a4\u30af\u30eb\u65e9\u304f\u3001\u3042\u308b\u3044\u306f\u4f55\u30b5\u30a4\u30af\u30eb\u9045\u304fThreshold Line\u306b\u9054\u3059\u308b\u304b\u306b\u6ce8\u76ee\u3057\u3066\u76f8\u5bfe\u5b9a\u91cf\u3059\u308b\u65b9\u6cd5\u3067\u3059\u30021\u30b5\u30a4\u30af\u30eb\u306e\u691c\u51fa\u306e\u9055\u3044\u30672\u500d\u91cf\u306e\u5dee\u3068\u306a\u308b\u3001\u3068\u3044\u3046\u7406\u8ad6\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002\u691c\u91cf\u7dda\u4f5c\u6210\u304c\u4e0d\u8981\u306a\u306e\u3067\u591a\u30b5\u30f3\u30d7\u30eb\u3092\u51e6\u7406\u3067\u304d\u308b\u3068\u3044\u3046\u30e1\u30ea\u30c3\u30c8\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u3068\u5185\u5728\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u907a\u4f1d\u5b50\u306ePCR\u52b9\u7387\u304c\u307b\u307c\u7b49\u3057\u3044\u3053\u3068\u3092\u78ba\u8a8d\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002 <\/p>\n","protected":false},"excerpt":{"rendered":"
\u907a\u4f1d\u5b50\u95a2\u9023\u6280\u8853\u306e\u767a\u9054\u306b\u5bc4\u3063\u3066\u3001\u5206\u5b50\u30ec\u30d9\u30eb\u306e\u5bdb\u89e3\u3092\u78ba\u8a8d\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u3063\u305f\u3002 \u3060\u304b\u3089\u3068\u8a00\u3063\u3066\u518d\u767a\u3057\u306a\u3044\u3068\u8a00\u3046\u8a33\u3067\u306f\u306a\u3044\u304c\u3001\u5206\u5b50\u30ec\u30d9\u30eb\u3067\u304c\u3093\u7d30\u80de\u304c\u306a\u304f\u306a\u3063\u305f\u3053\u3068\u3092\u78ba\u8a8d\u3067\u304d\u308b\u306e\u306f\u5fc3\u5f37\u3044\u3002<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[],"class_list":["post-133","post","type-post","status-publish","format-standard","hentry","category-6"],"_links":{"self":[{"href":"https:\/\/haruko.km2m.net\/index.php?rest_route=\/wp\/v2\/posts\/133","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/haruko.km2m.net\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/haruko.km2m.net\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/haruko.km2m.net\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/haruko.km2m.net\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=133"}],"version-history":[{"count":0,"href":"https:\/\/haruko.km2m.net\/index.php?rest_route=\/wp\/v2\/posts\/133\/revisions"}],"wp:attachment":[{"href":"https:\/\/haruko.km2m.net\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=133"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/haruko.km2m.net\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=133"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/haruko.km2m.net\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=133"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}